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Image Search Results
Journal: Frontiers in Cardiovascular Medicine
Article Title: Experimental renal transplantation in rats improves cardiac dysfunction caused by chronic kidney disease while LVH persists
doi: 10.3389/fcvm.2023.1200323
Figure Lengend Snippet: Changes in markers of cardiac hypertrophy, fibrosis and inflammation in CKD and after renal function restoration by transplantation. mRNA expression of BNP ( A ), FGF23 ( B ), FGFR4 ( C ), fibronectin ( D ), CTGF ( K ) and IL-6 ( L ) was analyzed in hearts from sham, SNx and SNx rat with renal transplant using Real-time PCR. Cardiac fibronectin ( E ) was analyzed by immunohistochemistry using a semi-quantitative score and examples of cardiac fibronectin staining in controls and SNx rats are shown ( F , brown staining). The myofibroblast marker collagen 1 was analyzed semi-quantitatively in heart sections ( G ). Representative pictures of collagen staining in hearts of sham control, SNx and RTx rats are shown ( H , brown staining). Perivascular microscarring was analyzed using a semi-quantitative score ( I ) and collagen 1 stained sections ( J ). Cardiac macrophages were analyzed by counting of ED1-positive cells detected using immunohistochemistry ( M ). * p < 0.05 vs. control, ** p < 0.01 vs. control, *** p < 0.001 vs. control, **** p < 0.0001, # p < 0.05 vs. SNx, ## p < 0.01 vs. SNx, ### p < 0.001 vs. SNx.
Article Snippet: The following primary antibodies were used: a polyclonal rabbit anti-fibronectin antibody (ab2413, Abcam, Cambridge, GB); a polyclonal rabbit anti-collagen 1 antibody (Novus Biologicals, LLC, Centennial, CO, USA) and a
Techniques: Transplantation Assay, Expressing, Real-time Polymerase Chain Reaction, Immunohistochemistry, Staining, Marker, Control